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Literature summary extracted from

  • Sadahiro, J.; Mori, H.; Saburi, W.; Okuyama, M.; Kimura, A.
    Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth (2015), Biochem. Biophys. Res. Commun., 456, 500-505.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.2 recombinant expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)-CodonPlus-RIL Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.2 extracellular the extracellular enzyme is secreted, liberation from the spheroplast cells Gluconobacter oxydans
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2.4.1.2 intracellular cell surface, the cell-associated form (DDint) appears after depletion of glucose from the medium Gluconobacter oxydans 5622
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2.4.1.2 additional information extracellular and cell-associated forms of the enzyme change their localization depending on the cell growth. Genomic southern blot, peptide mass fingerprinting and reaction product-pattern analyses reveal that the enzyme forms are structurally identical Gluconobacter oxydans
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Organism

EC Number Organism UniProt Comment Textmining
2.4.1.2 Gluconobacter oxydans A0A0M5MSC0
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2.4.1.2 Gluconobacter oxydans ATCC 11894 A0A0M5MSC0
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.2 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-CodonPlus-RIL by nickel affinity chromatography, dialysis, and ultrafiltration Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.2 additional information substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose Gluconobacter oxydans ?
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 ?
2.4.1.2 additional information substrate is maltotetraose, product analysis from reactions of intra- and extracellular enzymes, overview. Main substrates are maltotriose and maltopentaose Gluconobacter oxydans ATCC 11894 ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.2 DDase
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 Gluconobacter oxydans
2.4.1.2 dextran dextrinase
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 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.2 37
-
 assay at Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.2 5.4
-
 assay at Gluconobacter oxydans

General Information

EC Number General Information Comment Organism
2.4.1.2 physiological function the enzyme synthesizes dextran from the starch hydrolysate, dextrin Gluconobacter oxydans